[Insect-resistant transgenic poplar expressing AaIT gene].
Identifieur interne : 004752 ( Main/Exploration ); précédent : 004751; suivant : 004753[Insect-resistant transgenic poplar expressing AaIT gene].
Auteurs : N F Wu [République populaire de Chine] ; Q. Sun ; B. Yao ; Y L Fan ; H Y Rao ; M R Huang ; M X WangSource :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology [ 1000-3061 ] ; 2000.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Scorpion Venoms.
- chemical : Insecticides.
- Animals, Blotting, Southern, Insecta, Plants, Genetically Modified, Polymerase Chain Reaction, Transformation, Genetic.
Abstract
Insect-specific scorpion neurotoxin AaIT gene inserted into a binary vector was transferred into a hybrid poplar clone N-106(P. deltoides x P. simonii) growing in the Southern of China. We obtained sixty-two regenerated plants by Agrobacterium tumefaciens transferring system. PCR and PCR-Southern analysis showed that AaIT gene was incorporated into the genome of some recovered poplar plants. One of the transformed plants named A5 was significantly resistant to feeding by first instar larvae of Lymantria dispar, compared with the untransformed control plant. It caused a decrease in leaf consumption by larvae, a lower larval weight gain and a higher larval motality rate of Lymantria dispar. ELISA analysis proved that AaIT gene was expressed in this transfomed poplar plant.
PubMed: 10976310
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Insect-resistant transgenic poplar expressing AaIT gene].</title><author><name sortKey="Wu, N F" sort="Wu, N F" uniqKey="Wu N" first="N F" last="Wu">N F Wu</name><affiliation wicri:level="3"><nlm:affiliation>Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing.</nlm:affiliation><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><wicri:orgArea>Biotechnology Research Center, Chinese Academy of Agricultural Sciences</wicri:orgArea></affiliation></author><author><name sortKey="Sun, Q" sort="Sun, Q" uniqKey="Sun Q" first="Q" last="Sun">Q. Sun</name></author><author><name sortKey="Yao, B" sort="Yao, B" uniqKey="Yao B" first="B" last="Yao">B. Yao</name></author><author><name sortKey="Fan, Y L" sort="Fan, Y L" uniqKey="Fan Y" first="Y L" last="Fan">Y L Fan</name></author><author><name sortKey="Rao, H Y" sort="Rao, H Y" uniqKey="Rao H" first="H Y" last="Rao">H Y Rao</name></author><author><name sortKey="Huang, M R" sort="Huang, M R" uniqKey="Huang M" first="M R" last="Huang">M R Huang</name></author><author><name sortKey="Wang, M X" sort="Wang, M X" uniqKey="Wang M" first="M X" last="Wang">M X Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:10976310</idno><idno type="pmid">10976310</idno><idno type="wicri:Area/Main/Corpus">004839</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004839</idno><idno type="wicri:Area/Main/Curation">004839</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004839</idno><idno type="wicri:Area/Main/Exploration">004839</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Insect-resistant transgenic poplar expressing AaIT gene].</title><author><name sortKey="Wu, N F" sort="Wu, N F" uniqKey="Wu N" first="N F" last="Wu">N F Wu</name><affiliation wicri:level="3"><nlm:affiliation>Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing.</nlm:affiliation><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><wicri:orgArea>Biotechnology Research Center, Chinese Academy of Agricultural Sciences</wicri:orgArea></affiliation></author><author><name sortKey="Sun, Q" sort="Sun, Q" uniqKey="Sun Q" first="Q" last="Sun">Q. Sun</name></author><author><name sortKey="Yao, B" sort="Yao, B" uniqKey="Yao B" first="B" last="Yao">B. Yao</name></author><author><name sortKey="Fan, Y L" sort="Fan, Y L" uniqKey="Fan Y" first="Y L" last="Fan">Y L Fan</name></author><author><name sortKey="Rao, H Y" sort="Rao, H Y" uniqKey="Rao H" first="H Y" last="Rao">H Y Rao</name></author><author><name sortKey="Huang, M R" sort="Huang, M R" uniqKey="Huang M" first="M R" last="Huang">M R Huang</name></author><author><name sortKey="Wang, M X" sort="Wang, M X" uniqKey="Wang M" first="M X" last="Wang">M X Wang</name></author></analytic><series><title level="j">Sheng wu gong cheng xue bao = Chinese journal of biotechnology</title><idno type="ISSN">1000-3061</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Blotting, Southern (MeSH)</term><term>Insecta (MeSH)</term><term>Insecticides (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Scorpion Venoms (genetics)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Insectes (MeSH)</term><term>Insecticides (MeSH)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Technique de Southern (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Venins de scorpion (génétique)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Scorpion Venoms</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Insecticides</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Venins de scorpion</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Southern</term><term>Insecta</term><term>Plants, Genetically Modified</term><term>Polymerase Chain Reaction</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Insectes</term><term>Insecticides</term><term>Réaction de polymérisation en chaîne</term><term>Technique de Southern</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Insect-specific scorpion neurotoxin AaIT gene inserted into a binary vector was transferred into a hybrid poplar clone N-106(P. deltoides x P. simonii) growing in the Southern of China. We obtained sixty-two regenerated plants by Agrobacterium tumefaciens transferring system. PCR and PCR-Southern analysis showed that AaIT gene was incorporated into the genome of some recovered poplar plants. One of the transformed plants named A5 was significantly resistant to feeding by first instar larvae of Lymantria dispar, compared with the untransformed control plant. It caused a decrease in leaf consumption by larvae, a lower larval weight gain and a higher larval motality rate of Lymantria dispar. ELISA analysis proved that AaIT gene was expressed in this transfomed poplar plant.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10976310</PMID><DateCompleted><Year>2000</Year><Month>09</Month><Day>25</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-3061</ISSN><JournalIssue CitedMedium="Print"><Volume>16</Volume><Issue>2</Issue><PubDate><Year>2000</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Sheng wu gong cheng xue bao = Chinese journal of biotechnology</Title><ISOAbbreviation>Sheng Wu Gong Cheng Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Insect-resistant transgenic poplar expressing AaIT gene].</ArticleTitle><Pagination><MedlinePgn>129-33</MedlinePgn></Pagination><Abstract><AbstractText>Insect-specific scorpion neurotoxin AaIT gene inserted into a binary vector was transferred into a hybrid poplar clone N-106(P. deltoides x P. simonii) growing in the Southern of China. We obtained sixty-two regenerated plants by Agrobacterium tumefaciens transferring system. PCR and PCR-Southern analysis showed that AaIT gene was incorporated into the genome of some recovered poplar plants. One of the transformed plants named A5 was significantly resistant to feeding by first instar larvae of Lymantria dispar, compared with the untransformed control plant. It caused a decrease in leaf consumption by larvae, a lower larval weight gain and a higher larval motality rate of Lymantria dispar. ELISA analysis proved that AaIT gene was expressed in this transfomed poplar plant.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>N F</ForeName><Initials>NF</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Q</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Y L</ForeName><Initials>YL</Initials></Author><Author ValidYN="Y"><LastName>Rao</LastName><ForeName>H Y</ForeName><Initials>HY</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>M R</ForeName><Initials>MR</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>M X</ForeName><Initials>MX</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Sheng Wu Gong Cheng Xue Bao</MedlineTA><NlmUniqueID>9426463</NlmUniqueID><ISSNLinking>1000-3061</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007306">Insecticides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012604">Scorpion Venoms</NameOfSubstance></Chemical><Chemical><RegistryNumber>131092-86-9</RegistryNumber><NameOfSubstance UI="C065634">AaIT neurotoxin, Androctonus australis</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007313" MajorTopicYN="N">Insecta</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007306" MajorTopicYN="Y">Insecticides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012604" MajorTopicYN="N">Scorpion Venoms</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>9</Month><Day>8</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>9</Month><Day>30</Day><Hour>11</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>9</Month><Day>8</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10976310</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Fan, Y L" sort="Fan, Y L" uniqKey="Fan Y" first="Y L" last="Fan">Y L Fan</name><name sortKey="Huang, M R" sort="Huang, M R" uniqKey="Huang M" first="M R" last="Huang">M R Huang</name><name sortKey="Rao, H Y" sort="Rao, H Y" uniqKey="Rao H" first="H Y" last="Rao">H Y Rao</name><name sortKey="Sun, Q" sort="Sun, Q" uniqKey="Sun Q" first="Q" last="Sun">Q. Sun</name><name sortKey="Wang, M X" sort="Wang, M X" uniqKey="Wang M" first="M X" last="Wang">M X Wang</name><name sortKey="Yao, B" sort="Yao, B" uniqKey="Yao B" first="B" last="Yao">B. Yao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wu, N F" sort="Wu, N F" uniqKey="Wu N" first="N F" last="Wu">N F Wu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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